Western blot analysis of extracts of HeLa cells, using Phospho-PKR/EIF2AK2-T446 antibody (TA375789) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Western blot analysis of extracts of HeLa cells, using Phospho-PKR/EIF2AK2-T446 antibody (TA375789) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
PKR (EIF2AK2) Rabbit Polyclonal Antibody
TA375789
Overview
- SupplierOriGene
- Product NamePKR (EIF2AK2) Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5610
- Target nameEIF2AK2
- Target descriptioneukaryotic translation initiation factor 2 alpha kinase 2
- Target synonymsdouble stranded RNA activated protein kinase; eIF-2A protein kinase 2; EIF2AK1; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; LEUDEN; P1/eIF-2A protein kinase; p68 kinase; PKR; PPP1R83; PRKR; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-PKR/EIF2AK2-T446 Rabbit polyclonal Antibody
- ReactivityMouse
- UNSPSC12352203