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Plectin (PLEC) (C-term) Guinea Pig Polyclonal Antibody

BP5115
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetPLEC
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Plectin (PLEC) (C-term) Guinea Pig Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5339
  • Target name
    PLEC
  • Target description
    plectin
  • Target synonyms
    EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; hemidesmosomal protein 1; LGMD2Q; LGMDR17; PCN; PLEC1; PLEC1b; plectin; plectin 1, intermediate filament binding protein 500kDa; PLTN
  • Host
    Guinea Pig
  • Protein IDQ15149
  • Protein Name
    Plectin
  • Scientific Description
    Plectin (PLEC) (C-term) guinea pig polyclonal antibody, Serum
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Plectin using anti-Plectin antibody (PB9430). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plectin antigen affinity purified polyclonal antibody (Catalog # PB9430) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plectin at approximately 532 kDa. The expected band size for Plectin is at 532 kDa.
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