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ProductsBack/PLET1 / C11orf34 Antibody (aa81-130)

PLET1 / C11orf34 Antibody (aa81-130)

LS-C169950
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPLET1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PLET1 / C11orf34 Antibody (aa81-130)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID349633
  • Target name
    PLET1
  • Target description
    placenta expressed transcript 1
  • Target synonyms
    C11orf34; placenta-expressed transcript 1 protein
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PLET1 using anti-PLET1 antibody (A14923-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLET1 antigen affinity purified polyclonal antibody (Catalog # A14923-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLET1 at approximately 28 kDa. The expected band size for PLET1 is at 23,25 kDa.
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Anti-PLET1 Antibody Picoband(r)A14923-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPLET1
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Product group Antibodies
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PLET1 / C11orf34 Antibody (HRP)LS-C378933
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPLET1
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Western Blot Positive WB detected in Recombinant protein All lanes: PLET1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 24.4 kDa Observed band size: 28 kDa
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ReactivityHuman
TargetPLET1
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