POLA1 Antibody

Cusabio

POLA1 Antibody

20

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

POLA1

DNA polymerase alpha 1, catalytic subunit

DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; NSX; p180; POLA; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); VEODS

Rabbit

IgG

DNA polymerase alpha catalytic subunit

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3 exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Wong S.W., EMBO J. 7:37-47(1988). Pearson B.E., Mol. Cell. Biol. 11:2081-2095(1991). Hsi K.-L., Nucleic Acids Res. 18:6231-6237(1990).

Human

-20°C or -80°C

12352203