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Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 μg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4o followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 μg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4o followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Western blot using GeneTex's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead). Approximately 35 μg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4o followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated rabbit anti-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

POLK antibody

GTX48495
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPOLK
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Overview

  • Supplier
    GeneTex
  • Product Name
    POLK antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ELISA: 1:2000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51426
  • Target name
    POLK
  • Target description
    DNA polymerase kappa
  • Target synonyms
    DINB1, DINP, POLQ, DNA polymerase kappa, polymerase (DNA directed) kappa
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UBT6
  • Protein Name
    DNA polymerase kappa
  • Scientific Description
    POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3-5 proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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