Poly(A) Polymerase, Yeast

Applied Biological Materials

Poly(A) Polymerase, Yeast

9

Research Use Only

Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3 ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5-triphosphate (3-dATP) for ATP results in addition of a single dA residue to the 3-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.

-20°C

12352204