Immunohistochemistry showing GeneTex's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20oC. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20oC. A biotinylated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).
Immunohistochemistry showing GeneTex's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20oC. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20oC. A biotinylated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).
PPAR alpha antibody
GTX28934
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPpara
Overview
- SupplierGeneTex
- Product NamePPAR alpha antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1-5 microg/mL. IHC-P: 1:100-:300. ELISA: 1:75000-1:125000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID19013
- Target namePpara
- Target descriptionperoxisome proliferator activated receptor alpha
- Target synonyms4933429D07Rik, Nr1c1, PPAR-alpha, PPARalpha, Ppar, peroxisome proliferator-activated receptor alpha, nuclear receptor subfamily 1 group C member 1
- HostRabbit
- IsotypeIgG
- Protein IDP23204
- Protein NamePeroxisome proliferator-activated receptor alpha
- Scientific DescriptionThe Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Lipid Messenger Phosphatidylinositol-4,5-Bisphosphate Is Increased by Both PPARalpha Activators and Inhibitors: Relevance for Intestinal Cell Differentiation.Read this paper
- When Activator and Inhibitor of PPARalpha Do the Same: Consequence for Differentiation of Human Intestinal Cells. Cizkova K et al., 2021 Sep 17, BiomedicinesRead this paper
- A novel supplement with yeast beta-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Nehmi VA et al., 2021 Sep, J Integr MedRead this paper
- Yulink, predicted from evolutionary analysis, is involved in cardiac function. Kuo MW et al., 2021 Jan 11, J Biomed SciRead this paper
- Expression of cytochrome P450 epoxygenases and soluble epoxide hydrolase is regulated by hypolipidemic drugs in dose-dependent manner. Cizkova K, 2018 Sep 15, Toxicol Appl PharmacolRead this paper
- Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig. Grossini E et al., 2014 Jul, J EndocrinolRead this paper
Datasheet
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