Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody (GTX28934). Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 microg/mL overnight at 4oC. Secondary antibody: Alexa FluorR 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa FluorR 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.
Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody (GTX28934). Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 microg/mL overnight at 4oC. Secondary antibody: Alexa FluorR 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa FluorR 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.
PPAR alpha antibody
GTX28934
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPpara
Overview
- SupplierGeneTex
- Product NamePPAR alpha antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1-5 microg/mL. IHC-P: 1:100-:300. ELISA: 1:75000-1:125000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID19013
- Target namePpara
- Target descriptionperoxisome proliferator activated receptor alpha
- Target synonyms4933429D07Rik, Nr1c1, PPAR-alpha, PPARalpha, Ppar, peroxisome proliferator-activated receptor alpha, nuclear receptor subfamily 1 group C member 1
- HostRabbit
- IsotypeIgG
- Protein IDP23204
- Protein NamePeroxisome proliferator-activated receptor alpha
- Scientific DescriptionThe Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cizkova K, Koubova K, Tauber Z. Lipid Messenger Phosphatidylinositol-4,5-Bisphosphate Is Increased by Both PPARα Activators and Inhibitors: Relevance for Intestinal Cell Differentiation. Biology (Basel). 2022,11(7). doi: 10.3390/biology11070997Read this paper
- Cizkova K, Foltynkova T, Hanyk J, et al. When Activator and Inhibitor of PPARα Do the Same: Consequence for Differentiation of Human Intestinal Cells. Biomedicines. 2021,9(9). doi: 10.3390/biomedicines9091255Read this paper
- Nehmi VA, Murata GM, Moraes RCM, et al. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. J Integr Med. 2021,19(5):439-450. doi: 10.1016/j.joim.2021.05.002Read this paper
- Kuo MW, Tsai HH, Wang SH, et al. Yulink, predicted from evolutionary analysis, is involved in cardiac function. J Biomed Sci. 2021,28(1):7. doi: 10.1186/s12929-020-00701-7Read this paper
- Cizkova K. Expression of cytochrome P450 epoxygenases and soluble epoxide hydrolase is regulated by hypolipidemic drugs in dose-dependent manner. Toxicol Appl Pharmacol. 2018,355:156-163. doi: 10.1016/j.taap.2018.06.025Read this paper
- Grossini E, Prodam F, Walker GE, et al. Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig. J Endocrinol. 2014,222(1):137-49. doi: 10.1530/JOE-14-0170Read this paper
Datasheet
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