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ProductsBack/PPP2R5D Antibody - C-terminal region (OAGA02743)

PPP2R5D Antibody - C-terminal region (OAGA02743)

OAGA02743
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPP2R5D
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PPP2R5D Antibody - C-terminal region (OAGA02743)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. PBS with 10% Glycerol (pH7) and 0.01% Thimerosal.
  • Gene ID5528
  • Target name
    PPP2R5D
  • Target description
    protein phosphatase 2 regulatory subunit B'delta
  • Target synonyms
    B56D; B56delta; MRD35; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP2R5D using anti-PPP2R5D antibody (A06450-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5D antigen affinity purified polyclonal antibody (Catalog # A06450-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5D at approximately 74 kDa. The expected band size for PPP2R5D is at 70 kDa.
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