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IHC analysis of FDCSP using anti-FDCSP antibody (A13093-1).
Product group Antibodies
Boster Bio
Anti-FDCSP AntibodyA13093-1
Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human U2OS whole cell lysates, Lane 8: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.
Product group Antibodies
Boster Bio
Anti-TMEM199 AntibodyA14686-1
Western blot analysis of CCDC36 using anti-CCDC36 antibody (A14775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC36 antigen affinity purified polyclonal antibody (Catalog # A14775) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC36 at approximately 66KD. The expected band size for CCDC36 is at 66KD.
Product group Antibodies
Boster Bio
Anti-CCDC36 AntibodyA14775
Western blot analysis of TMEM240 using anti-TMEM240 antibody (A14889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: NIH3T3 whole Cell lysates, Lane 3: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM240 antigen affinity purified polyclonal antibody (Catalog # A14889) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM240 at approximately 19KD. The expected band size for TMEM240 is at 19KD.
Product group Antibodies
Boster Bio
Anti-TMEM240 AntibodyA14889
Western blot analysis of RNF186 using anti-RNF186 antibody (A15259-1).
Product group Antibodies
Boster Bio
Anti-RNF186 AntibodyA15259-1
Western blot analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1).
Product group Antibodies
Boster Bio
Anti-KBTBD2 AntibodyA15277-1
Anti-peeling Slide
Product group Antibodies
Boster Bio
Anti-peeling SlideAR1065
Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-2).
Product group Antibodies
Boster Bio
Anti-TNF alpha Picoband AntibodyA00002-2
Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
Product group Antibodies
Boster Bio
Anti-TNF alpha AntibodyA00002-3
IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-beta Catenin AntibodyA00004
Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005).
Product group Antibodies
Boster Bio
Anti-BRCA1 Picoband AntibodyA00005
Western blot analysis of PTEN using anti-PTEN antibody (A00006-1).
Product group Antibodies
Boster Bio
Anti-PTEN AntibodyA00006-1
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