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The Binding Activity of Labetuzumab govitecan (Synonyms: IMMU 130; hMN-14-SN-38) with Anti-SN38 antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Labetuzumab govitecan (Synonyms: IMMU 130; hMN-14-SN-38) at 2 microg/mL can bind Anti-SN38 antibody, the EC50 is 3.326 to 4.721 ng/mL.
Product group Antibodies
Cusabio
SN38 Recombinant Monoclonal AntibodyCSB-RA187707I1M
ApplicationsELISA
ReactivityHuman
Overlay Peak curve showing RAJI cells stained with CSB-RA188599A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
LCP1 Recombinant Monoclonal AntibodyCSB-RA188599A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetLCP1
IHC image of CSB-RA191819A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.78% DAB.
Product group Antibodies
Cusabio
RAN Recombinant Monoclonal AntibodyCSB-RA191819A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetRAN
Western Blot Positive WB detected in: SY5Y whole cell lysate,U251 whole cell lysate,MCF7 whole cell lysate,K562 whole cell lysate,Mouse Brain tissue lysate,Mouse Brain tissue lysate All lanes: EGR2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa
Product group Antibodies
Cusabio
EGR2 Recombinant Monoclonal AntibodyCSB-RA196762A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetEGR2
IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.
Product group Antibodies
Cusabio
PRKAR2A Recombinant Monoclonal AntibodyCSB-RA197122A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPRKAR2A
Western Blot Positive WB detected in: HEPG2 whole cell lysate All lanes: Collagen XVII antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 150 kDa Observed band size: 150 kDa
Product group Antibodies
Cusabio
COL17A1 Recombinant Monoclonal AntibodyCSB-RA217204A0HU
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetCOL17A1
Western Blot Positive WB detected in: K562 whole cell lysate,U937 whole cell lysate All lanes: Ikaros antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Product group Antibodies
Cusabio
CA1 Recombinant Monoclonal AntibodyCSB-RA217389A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCA1
Western Blot Positive WB detected in: THP-1 whole cell lysate All lanes: Caveolin-1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
Product group Antibodies
Cusabio
CAV1 Recombinant Monoclonal AntibodyCSB-RA228590A0HU
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetCAV1
Overlay Peak curve showing Hela cells stained with CSB-RA241995A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
GFAP Recombinant Monoclonal AntibodyCSB-RA241995A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetGFAP
Overlay Peak curve showing Jurkat cells stained with CSB-RA244537A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
CD247 Recombinant Monoclonal AntibodyCSB-RA244537A0HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCD247
IHC image of CSB-RA24801:50A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.59% DAB.
Product group Antibodies
Cusabio
PAX8 Recombinant Monoclonal AntibodyCSB-RA248028A0HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPAX8
Western Blot Positive WB detected in: HT29 whole cell lysate, U87 whole cell lysate,Hela whole cell lysate,A549 whole cell lysate,HEPG2 whole cell lysate All lanes: Syntenin antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
Product group Antibodies
Cusabio
SDCBP Recombinant Monoclonal AntibodyCSB-RA248860A0HU
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetSDCBP
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