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Overlay Peak curve showing U937 cells stained with CSB-RA582227A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCXCL8
IHC image of CSB-RA582749A0HU diluted at 1:50 and staining in paraffin-embedded human pancreati tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.34% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetKRT10
IHC image of CSB-RA583078A0HU diluted at 1:50 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.24% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetINMT
IHC image of CSB-RA588766A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.23% DAB.
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetERBB2
IHC image of CSB-RA588962A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.76% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetL1CAM
IHC image of CSB-RA594821A0HU diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.43% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSOCS7
The Binding Activity of Human CDH17 with Anti-CDH17 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CDH17 (CSB-MP613267HU) at 2 microg/mL can bind Anti-CDH17 recombinant antibody, the EC50 is 3.095-4.451 ng/mL.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCDH17
Overlay Peak curve showing A549 cells stained with CSB-RA614787A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetSCGB1A1
IHC image of CSB-RA614975MA1HU diluted at 1:150 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDPYSL2
Immunofluorescence staining of CSB-RA619082MA1HU cell with CSB-RA013714MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetNTRK2
The Binding Activity of Human CLDN6 with Anti-CLDN6 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 microg/mL can bind Anti-CLDN6 recombinant antibody. The EC50 is 12.95-16.18 ng/mL. The VLPs (CSB-MP3838) is negative control.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetCLDN6
IHC image of CSB-RA005728MA1HU diluted at 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCOL1A2