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The Binding Activity of Human TF with Anti-TF Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TF (CSB-MP007928HU2) at 2 microg/mL can bind Anti-TF recombinant antibody. The EC50 is 1.434-1.635 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetF3
Immunofluorescence staining of Hela cell with CSB-RA008543MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetFCGR3A
IHC image of CSB-RA008642MA1HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetFGFR1
Overlay Peak curve showing MCF7 cells stained with CSB-RA008646MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetFGFR3
IHC image of CSB-RA008732MA1HU diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetFLT1
Overlay Peak curve showing 3T3 cells stained with CSB-RA008759MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetFN1
The Binding Activity of Cynomolgus FOLR1 with Anti-FOLR1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus FOLR1(CSB-MP5002MOV) at 2 microg/mL can bind Anti-FOLR1 recombinant antibody. The EC50 is 2.900-3.544 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman, Monkey
TargetFOLR1
Western Blot Positive WB detected in: U251 whole cell lysate,JK whole cell lysate All lanes: FTH1 antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetFTH1
Western Blot Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,JK whole cell lysate All lanes: GAPDH antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGAPDH
Western Blot Positive WB detected in: MCF7 whole cell lysate,Mouse Brain tissue lysate,Rat Brain tissue lysate All lanes: GFAP antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 55 kDa
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGFAP
The Binding Activity of Macaca fascicularis GIPR with Anti-GIPR recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GIPR (CSB-YP4825MOV) at 2 microg/mL can bind Anti-GIPR recombinant antibody. The EC50 is 12.66-13.86 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman, Monkey
TargetGIPR
The Binding Activity of Mouse Gipr with Anti-Mouse Gipr recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Gipr (CSB-MP009438MO1) at 2 microg/mL can bind Anti-Mouse Gipr recombinant antibody , the EC50 is 8.622-11.36 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityMouse, Rat
TargetGipr