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IHC image of CSB-RA011048MA1HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIFNB1
Overlay Peak curve showing Hela cells stained with CSB-RA011087MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 008 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetIGF1R
IHC image of CSB-RA011580MA1HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL10
The Binding Activity of Human IL12RB1 with Anti-IL12RB1 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL12RB1(CSB-MP011588HU3) at 2 microg/mL can bind Anti-IL12RB1 recombinant antibody. The EC50 is 1.578-2.143 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetIL12RB1
IHC image of CSB-RA011591MA1HU diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL13RA1
Immunofluorescence staining of MCF7 cell with CSB-RA011593MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetIL15
IHC image of CSB-RA011614MA1HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL1B
Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA011621MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetIL1R1
The Binding Activity of Human IL2RA with Anti-IL2RA Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA (CSB-MP011649HU3) at 2 microg/mL can bind Anti-IL2RA recombinant antibody , the EC50 is 2.463-3.353 ng/mL.
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetIL2RA
The Binding Activity of Rhesus macaque IL2RG with Anti-IL2RG recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque IL2RG (CSB-MP2765MOW) at 2 microg/mL can bind Anti-IL2RG recombinant antibody. The EC50 is 33.73-41.04 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman, Monkey
TargetIL2RG
The Binding Activity of Human IL6 with Anti-IL6 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL6 (CSB-YP011664HU) at 2 microg/mL can bind Anti-IL6 recombinant antibody. The EC50 is 35.80-41.82 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetIL6
IHC image of CSB-RA011664MA2HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIL6