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IHC image of CSB-RA011868MA1HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetITGA5
Immunofluorescence staining of NIH/3T3 cell with CSB-RA011877MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman, Mouse
TargetITGAV
Overlay Peak curve showing A549 cells stained with CSB-RA011887MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetITGB4
IHC image of CSB-RA012145MA1HU diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetKDR
IHC image of CSB-RA012352MA1HU diluted at 1 : 100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetKIR2DL1
The Binding Activity of Human KIR3DL2 with Anti-KIR3DL2 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human KIR3DL2(CSB-MP012365HU1) at 2 microg/mL can bind Anti-KIR3DL2 recombinant antibody , the EC50 is 14.18-23.93 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetKIR3DL2
IHC image of CSB-RA012375MA1HU diluted at 1 : 100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetKIT
IHC image of CSB-RA012719MA1HU diluted at 1 : 100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetLAG3
Overlay Peak curve showing 3T3 cells stained with CSB-RA012882MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 007 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetLGALS1
Western Blot Positive WB detected in: PC-3 whole cell lysate, MCF7 whole cell lysate, Hela whole cell lysate, A431 whole cell lysate All lanes: LGALS3 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa
Product group Antibodies
Cusabio
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetLGALS3
Overlay Peak curve showing MCF7 cells stained with CSB-RA013481MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetMAPT
Immunofluorescence staining of SH-SY5Y cell with CSB-RA013714MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetMET