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Immunofluorescence staining of hela cell with CSB-RA013826MA1HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
Product group Antibodies
Cusabio
MIF Recombinant Monoclonal AntibodyCSB-RA013826MA1HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetMIF
IHC image of CSB-RA014781MA1HU diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
MRAS Recombinant Monoclonal AntibodyCSB-RA014781MA1HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMRAS
IHC image of CSB-RA015007MA4HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
MS4A1 Recombinant Monoclonal AntibodyCSB-RA015007MA4HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMS4A1
The Binding Activity of Human MYL9 with Anti-MYL9 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human MYL9 (CSB-YP015318HU) at 2 microg/mL can bind Anti-MYL9 recombinant antibody, the EC50 is 4.628-6.430 ng/mL.
Product group Antibodies
Cusabio
MYL9 Recombinant Monoclonal AntibodyCSB-RA015318MA1HU
ApplicationsELISA
ReactivityHuman
TargetMYL9
Overlay Peak curve showing PC3 cells stained with CSB-RA015779MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
NGF Recombinant Monoclonal AntibodyCSB-RA015779MA1HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetNGF
Overlay Peak curve showing PC3 cells stained with CSB-RA016091MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
NRP1 Recombinant Monoclonal AntibodyCSB-RA016091MA1HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetNRP1
The Binding Activity of Human OSM with Anti-OSM Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human OSM (CSB-MP017260HU1) at 2 microg/mL can bind Anti-OSM recombinant antibody, the EC50 is 3.048-3.860 ng/mL.
Product group Antibodies
Cusabio
OSM Recombinant Monoclonal AntibodyCSB-RA017260MA1HU
ApplicationsELISA
ReactivityHuman
TargetOSM
IHC image of CSB-RA017450A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.62% DAB.
Product group Antibodies
Cusabio
PXN Recombinant Monoclonal AntibodyCSB-RA017450A0HU
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPXN
Overlay Peak curve showing JK cells stained with CSB-RA017621MA1HU (red line) at 1:300. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
PCNA Recombinant Monoclonal AntibodyCSB-RA017621MA1HU
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetPCNA
Western Blot Positive WB detected in: PC-3 whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate All lanes: PDPN antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa
Product group Antibodies
Cusabio
PDPN Recombinant Monoclonal AntibodyCSB-RA017739MA1HU
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPDPN
Western Blot Positive WB detected in: THP-1 whole cell lysate, JK whole cell lysate All lanes: PECAM1 antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 82-140 kDa Observed band size: 140 kDa
Product group Antibodies
Cusabio
PECAM1 Recombinant Monoclonal AntibodyCSB-RA017767MA1HU
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetPECAM1
Immunofluorescence staining of hela cell with CSB-RA018122MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Product group Antibodies
Cusabio
PLAUR Recombinant Monoclonal AntibodyCSB-RA018122MA1HU
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetPLAUR
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