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Overlay Peak curve showing 293 cells stained with CSB-RA019049MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetPTPRC
Overlay Peak curve showing Hela cells stained with CSB-RA019677MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetRHD
IHC image of CSB-RA020888MA1HU diluted at 1:300 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Human IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSDC1
IHC image of CSB-RA021369MA1HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSLAMF7
IHC image of CSB-RA021912MA1HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSNCA
IHC image of CSB-RA021915MA1HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSNCG
IHC image of CSB-RA022332MA1HU diluted at 1:50 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSNRPD1
Immunofluorescence staining of Hela cell with CSB-RA022397MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetSOD1
Overlay Peak curve showing A549 cells stained with CSB-RA022603MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1microg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1microg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product group Antibodies
Cusabio
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetSPP1
The Binding Activity of Human STAT3 with Anti-STAT3 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human STAT3 (CSB-BP022812HU(A4)j7) at 2 microg/mL can bind Anti-STAT3 recombinant antibody , the EC50 is 41.31-75.59 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetSTAT3
Western Blot Positive WB detected in: A549 whole cell lysatee All lanes: SYP antibody at 1:500 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Product group Antibodies
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSYP
The Binding Activity of Human TFRC with Anti-TFRC Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TFRC (CSB-MP3648HU) at 2 microg/mL can bind Anti-TFRC recombinant antibody , the EC50 is 3.305-8.220 ng/mL.
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetTFRC