Bio-Connect

Primary

37 to 48 of 5.028 products
Sort by
IHC analysis of Rad51 using anti-Rad51 antibody (A00088). Rad51 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad51 Antibody (A00088) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
IHC analysis of PDGFRB using anti-PDGFRB antibody (A00096-1). PDGFRB was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PDGFRB Antibody (A00096-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Western blot analysis of FGFR1 using anti-FGFR1 antibody (A00098).
Product group Antibodies
Boster Bio
Western blot analysis of IL1 beta using anti-IL1 beta antibody (A00101-1).
Product group Antibodies
Boster Bio
Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate, Lane 2: rat spleen tissue lysate, Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
Product group Antibodies
Boster Bio
Western blot analysis of IL6 using anti-IL6 antibody (A00102-2).
Product group Antibodies
Boster Bio
Western blot analysis of HLA-DQB1 using anti-HLA-DQB1 antibody (A00106-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DQB1 antigen affinity purified polyclonal antibody (Catalog # A00106-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DQB1 at approximately 30KD. The expected band size for HLA-DQB1 is at 30KD.
Product group Antibodies
Boster Bio
Western blot analysis of PD-L1/B7-H1 using anti-PD-L1/B7-H1 antibody (A00109).
Product group Antibodies
Boster Bio
Western blot analysis of SP1 using anti-SP1 antibody (A00110-1).
Product group Antibodies
Boster Bio
Western blot analysis of IL33 using anti-IL33 antibody (A00113-1).
Product group Antibodies
Boster Bio
IHC analysis of IL33 using anti-IL33 antibody (A00113-2).
Product group Antibodies
Boster Bio
Western blot analysis of IL33 using anti-IL33 antibody (A00113).
Product group Antibodies
Boster Bio