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Product group Antibodies
AdipoGen Life Sciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA
ReactivityVirus
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Figure 1: Immunoblot analysis of beta-tubulin protein glutamylation using anti-beta-Tubulin, mAb (AXO45) (Prod. No. AG-20B-0085). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases, TTLL4 and TTLL6 and are run on a 10% SDS-PAGE (specific protocol to separate alpha- and beta-tubulin (Magiera & Janke, 2013)). The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the AXO45 (1:1000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In all the cell extracts, AXO45 specifically detects beta-tubulin, with no cross-reactivity to alpha-tubulin. Glutamylation added by either TTLL4 or TTLL6 appears to not affect this detection. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
Product group Antibodies
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Other Species
Product group Antibodies
AdipoGen Life Sciences
ApplicationsNeutralisation/Blocking
Product group Antibodies
AdipoGen Life Sciences
ApplicationsNeutralisation/Blocking
Product group Antibodies
AdipoGen Life Sciences
ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
ReactivityHuman
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PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (b
Product group Antibodies
AdipoGen Life Sciences
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD1
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PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (b
Product group Antibodies
AdipoGen Life Sciences
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD274
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PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (b
Product group Antibodies
AdipoGen Life Sciences
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIDH1
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anti-Poly(ADP-ribose) [PAR], mAb (10H)
Product group Antibodies
AdipoGen Life Sciences
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityDrosophila, Human, Mouse, Rat
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Product group Antibodies
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Porcine, Rabbit, Sheep
TargetVASP
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Western Blot using anti-Survival Motor Neuron Protein (human), mAb (7B10) (Prod. No. AG-20T-0003) on 15microg of human platelet lysate as a positive control (15microg). ECL detection.
Product group Antibodies
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetSMN1
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Immunofluorescence using anti-PARP-10 [ARTD10] (human), mAb (5H11). Method: T47D mammary carcinoma cells were stained by indirect immunofluorescence and analysed. Prior to fixation and staining, the cells were treated with or without 50nm leptomyci
Product group Antibodies
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPARP10
  • SizePrice