Immunofluorescence staining of A549 cells with CSB-PA03025A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PRMT1 Antibody

CSB-PA03025A0RB

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

ReactivityHuman, Mouse

TargetPRMT1

Overview

Supplier
Cusabio
Product Name
PRMT1 Antibody
Delivery Days Customer
20
Applications
ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID3276
Target name
PRMT1
Target description
protein arginine methyltransferase 1
Target synonyms
ANM1; HCP1; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; HRMT1L2; interferon receptor 1-bound protein 4; IR1B4; protein arginine N-methyltransferase 1
Host
Rabbit
Isotype
IgG
Protein IDQ99873
Protein Name
Protein arginine N-methyltransferase 1
Scientific Description
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:16879614, PubMed:26876602). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 Arg-4 (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
Reactivity
Human, Mouse
Storage Instruction
-20°C or -80°C
UNSPSC
41116161

PRMT1 Antibody

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