WB analysis of 20 ug of T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone for 1 hour using GTX22765 Progesterone Receptor antibody [Alpha PR6]. Dilution : 1 microg/ml
![ICC/IF analysis of T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right) using GTX22765 Progesterone Receptor antibody [Alpha PR6]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes](https://www.genetex.com/upload/website/prouct_img/normal/GTX22765/GTX22765_408_ICC-IF_w_23060620_281.webp "ICC/IF analysis of T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right) using GTX22765 Progesterone Receptor antibody [Alpha PR6]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes")
![IHC-P analysis of human uterus tissue using GTX22765 Progesterone Receptor antibody [Alpha PR6]. Antigen retrieval : 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX22765/GTX22765_953_IHC-P_w_23060620_462.webp "IHC-P analysis of human uterus tissue using GTX22765 Progesterone Receptor antibody [Alpha PR6]. Antigen retrieval : 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Dilution : 1:20")
WB analysis of 20 ug of T47D cell lysates, untreated (-) or stimulated (+) with 100 nm promegestone for 1 hour using GTX22765 Progesterone Receptor antibody [Alpha PR6]. Dilution : 1 microg/ml
Progesterone Receptor antibody [Alpha PR6]
GTX22765
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Chicken, Guinea Pig, Human, Mammals, Mouse, Primate, Rabbit, Rat, Sheep
TargetPGR
Overview
- SupplierGeneTex
- Product NameProgesterone Receptor antibody [Alpha PR6] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody does detects the B form of the progesterone receptor. It does not cross-react with estrogen receptor or glucocorticoid receptor.
- Application Supplier NoteWB: 1 microg/ml. ICC/IF: 10 microg/ml. IHC-P: 5 microg/ml. IHC-Fr: 20 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAlpha PR6
- ConjugateUnconjugated
- Gene ID396198
- Target namePGR
- Target descriptionprogesterone receptor
- Target synonymsNR3C3; nuclear receptor subfamily 3 group C member 3; PR; progesterone receptor
- HostMouse
- IsotypeIgG2a
- Protein IDP07812
- Protein NameProgesterone receptor
- Scientific DescriptionThis gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]
- ReactivityBovine, Chicken, Guinea Pig, Human, Mammals, Mouse, Primate, Rabbit, Rat, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Effect of Sex Steroids and PGF(2alpha) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early-Mid Pregnancy.Read more
- Impact of perinatal bisphenol A and 17beta estradiol exposure: Comparing hormone receptor response. Leonel ECR et al., 2020 Jan 30, Ecotoxicol Environ SafRead more