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Raji cell lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raji cell lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raji cell lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

EP4 Polyclonal Antibody

BS-8538R
Bioss Antibodies
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPTGER4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Prostaglandin E Receptor EP4 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5734
  • Target name
    PTGER4
  • Target description
    prostaglandin E receptor 4
  • Target synonyms
    EP4; EP4R; PGE receptor, EP4 subtype; PGE2 receptor EP4 subtype; prostaglandin E receptor 4 (subtype EP4); prostaglandin E2 receptor EP4 subtype; prostanoid EP4 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35408
  • Protein Name
    Prostaglandin E2 receptor EP4 subtype
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Wang Y et al., 2019 Aug, Prostaglandins Other Lipid Mediat
    Read more
  • Microsomal Prostaglandin E Synthase-1 Facilitates an Intercellular Interaction between CD4+ T Cells through IL-1beta Autocrine Function in Experimental Autoimmune Encephalomyelitis. Takemiya T et al., 2017 Dec 19, Int J Mol Sci
    Read more
  • The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii). Hu R et al., 2016 Jul, Prostaglandins Other Lipid Mediat
    Read more

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Figure 1. Western blot analysis of PTGER4 using anti-PTGER4 antibody (A02153-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole Cell lysates, Lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER4 antigen affinity purified polyclonal antibody (Catalog # A02153-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER4 at approximately 53KD. The expected band size for PTGER4 is at 53KD.
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