Michaelis-Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125microM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis-Menten equation. Kinetic parameters were calculated at: Km= 0.087microM, Vmax = 4.44nMs-1, kcat= 148s-1, kcat/Km= 1.70 x 109M-1s-1.
Michaelis-Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125microM was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25°C. Initial velocities at each substrate concentration were plotted and fit to the Michaelis-Menten equation. Kinetic parameters were calculated at: Km= 0.087microM, Vmax = 4.44nMs-1, kcat= 148s-1, kcat/Km= 1.70 x 109M-1s-1.
NEDP1 [SENP8] (human) (rec.) (His)
SBB-DE0025
Overview
- SupplierSouth Bay Bio
- Product NameNEDP1 [SENP8] (human) (rec.) (His)
- Delivery Days Customer10
- CertificationResearch Use Only
- Estimated Purity>95%
- Protein IDQ96LD8
- Protein NameSentrin-specific protease 8
- Scientific DescriptionNEDP1 is a deconjugating enzyme with specificity towards NEDDylated substrates. NEDP1 discriminates between ubiquitinated and NEDDylated targets, despite similar C-terminal regions. NEDP1 is a protease which hydrolyzes the isopeptide bond between the C-terminus of NEDD8 and the respective lysine of the substrate protein to which the NEDD8 is covalently bound. NEDP1 is important in regulating cullin associated ubiquitin ligase enzymes such as those of the SCF (Skp-Cullin-Fbox) family and subsequently, it is important in regulating cell cycle progression and other biological functions. - Protein. Human NEDP1 (aa2-212)is fused at the N-terminus to a His-tag. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP. Purity: >95% (SDS-PAGE). NEDP1 is a deconjugating enzyme with specificity towards NEDDylated substrates. NEDP1 discriminates between ubiquitinated and NEDDylated targets, despite similar C-terminal regions. NEDP1 is a protease which hydrolyzes the isopeptide bond between the C-terminus of NEDD8 and the respective lysine of the substrate protein to which the NEDD8 is covalently bound. NEDP1 is important in regulating cullin associated ubiquitin ligase enzymes such as those of the SCF (Skp-Cullin-Fbox) family and subsequently, it is important in regulating cell cycle progression and other biological functions.
- Storage Instruction-80°C
- UNSPSC12352202