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TEV Protease, His

Catalog number: Z03030-1K
Brand: GenScript
Packing: 1000 IU
Price: € 70.00
Expected delivery time: 10 days
Quantity:
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Product specifications for - TEV Protease, His

Overview: 
Product group: Proteins / Signaling Molecule
Category: Bacterial / viral proteins
Properties: 
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352202
Storage instructions: Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20-¦C from date of receipt. Please avoid freeze-thaw cycles.
Scientific information: 
Scientific info: Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C, also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Additional information: 
Synonyms: Z03030-1K; GenScript