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Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.
Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.
Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.

UBE2D2 [UbcH5b] (human) (rec.) (untagged)

Research Use Only
SBB-CE0027
South Bay Bio
Protein IDP62837
Product group Proteins / Signaling Molecules
Price on request
Packing Size
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Overview

  • Supplier
    South Bay Bio
  • Product Name
    UBE2D2 [UbcH5b] (human) (rec.) (untagged)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Estimated Purity
    >95%
  • Protein IDP62837
  • Protein Name
    Ubiquitin-conjugating enzyme E2 D2
  • Scientific Description
    Protein. Human UBE2D2 (aa1-147)is untagged. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol (v/v), 2mM TCEP. Purity: >95% (SDS-PAGE). UBE2D2 (UbcH5b) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D2 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D2-Ub thioester. UBE2D2 is capable of associating with numerous known E3 ligases which target abnormal proteins for proteasomal degradation through polyubiquitination. UBE2D2 is also known to interact with Parkin and to be involved in PINK1 mediated mitophagy. - UBE2D2 (UbcH5b) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D2 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D2-Ub thioester. UBE2D2 is capable of associating with numerous known E3 ligases which target abnormal proteins for proteasomal degradation through polyubiquitination. UBE2D2 is also known to interact with Parkin and to be involved in PINK1 mediated mitophagy.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352202