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Signal to Background: The signal to background ratio was determined by 100% hydrolysis of either 50nM or 500nM Ubiquitin-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.
Signal to Background: The signal to background ratio was determined by 100% hydrolysis of either 50nM or 500nM Ubiquitin-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.
Signal to Background: The signal to background ratio was determined by 100% hydrolysis of either 50nM or 500nM Ubiquitin-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA.

Ubiquitin (human) (rec.) (Rhodamine 110)

Research Use Only
SBB-PS0001
South Bay Bio
Protein IDP0CG47
Product group Proteins / Signaling Molecules
Price on request
Packing Size
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Overview

  • Supplier
    South Bay Bio
  • Product Name
    Ubiquitin (human) (rec.) (Rhodamine 110)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Estimated Purity
    >99%
  • Protein IDP0CG47
  • Protein Name
    Polyubiquitin-B
  • Scientific Description
    Protein. Human ubiquitin (aa1-76)conjugated at the C-terminus to a quenched Rhodamine 110 dye. Source: E. coli. Formulation: Liquid. In 50mM MES pH 6.0. Purity: >99% (LCMS). Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes and an E3 ubiquitin ligase. This enzymatic cascade results in modification of a epsilon-amine of a lysine residue on a substrate protein. Substrates may either be mono- or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities. - Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes and an E3 ubiquitin ligase. This enzymatic cascade results in modification of a epsilon-amine of a lysine residue on a substrate protein. Substrates may either be mono- or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352202