PRSS3 / Trypsin 3 Antibody

LifeSpan BioSciences

PRSS3 / Trypsin 3 Antibody

23

Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96%, rat: 76%, mouse: 74%, dog: 72%. and chicken: 67%. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.

Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15 ug/ml (IC 50) under conditions in which the enzyme was present at 0.03 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme. Western blot:1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.. Neut, WB (1 - 2 µg/ml) Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 0.15 ug/ml (IC 50) under conditions in which the enzyme was present at 0.03 ug/ml and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme. Western blot:1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.

Western Blot, Neutralisation/Blocking

Research Use Only

Monoclonal

Unconjugated

...

PRSS3

serine protease 3

brain trypsinogen; mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine 3; protease, serine, 4 (trypsin 4, brain); PRSS4; T9; TRY3; TRY4; trypsin III; trypsin IV; trypsin-3; trypsinogen 4; trypsinogen 5; trypsinogen IV

Rat

IgG2a

Human

-20°C

12352203