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ProductsBack/PSENEN / PEN-2 Antibody (aa89-98, clone 12K165)

PSENEN / PEN-2 Antibody (aa89-98, clone 12K165)

LS-C182936
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetPSENEN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PSENEN / PEN-2 Antibody (aa89-98, clone 12K165)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (8 - 25 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12K165
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55851
  • Target name
    PSENEN
  • Target description
    presenilin enhancer, gamma-secretase subunit
  • Target synonyms
    ACNINV2; gamma-secretase subunit PEN-2; hematopoietic stem/progenitor cells protein MDS033; MDS033; MSTP064; PEN2; PEN-2; presenilin enhancer 2 homolog
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEN2 using anti-PEN2 antibody (M04504-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEN2 antigen affinity purified monoclonal antibody (Catalog # M04504-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEN2 at approximately 12 kDa. The expected band size for PEN2 is at 12 kDa.
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