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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PTBP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 60, 60 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PTBP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 60, 60 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PTBP1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 60, 60 kDa Observed band size: 58 kDa

PTBP1 Recombinant Monoclonal Antibody

CSB-RA987652A0HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPTBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTBP1 Recombinant Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8A1
  • Conjugate
    Unconjugated
  • Gene ID5725
  • Target name
    PTBP1
  • Target description
    polypyrimidine tract binding protein 1
  • Target synonyms
    57 kDa RNA-binding protein PPTB-1; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; HNRNPI; HNRNP-I; HNRPI; polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I); polypyrimidine tract-binding protein 1; pPTB; PTB; PTB-1; PTB2; PTB3; PTB4; PTB-T; RNA-binding protein
  • Isotype
    IgG
  • Protein IDP26599
  • Protein Name
    Polypyrimidine tract-binding protein 1
  • Scientific Description
    Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798). PTBP1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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