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Western Blot Positive WB detected in: Mouse brain tissue, Rat liver tissue All lanes: PTF1A antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Rat liver tissue All lanes: PTF1A antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Rat liver tissue All lanes: PTF1A antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa

PTF1A Antibody

CSB-PA018967LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPTF1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTF1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID256297
  • Target name
    PTF1A
  • Target description
    pancreas associated transcription factor 1a
  • Target synonyms
    bHLH transcription factor p48; bHLHa29; class A basic helix-loop-helix protein 29; class II bHLH protein PTF1A; exocrine pancreas-specific transcription factor p48; p48; p48 DNA-binding subunit of transcription factor PTF1; PACA; PAGEN2; pancreas specific transcription factor, 1a; pancreas transcription factor 1 subunit alpha; PTF1-p48
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7RTS3
  • Protein Name
    Pancreas transcription factor 1 subunit alpha
  • Scientific Description
    Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5-CANNTG-3. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTF1A using anti-PTF1A antibody (A03891-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: mouse SP2-0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTF1A antigen affinity purified polyclonal antibody (Catalog # A03891-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTF1A at approximately 48KD. The expected band size for PTF1A is at 35KD.
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