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PTP1B Antibody (clone OTI1D10)

LS-C173186
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTPN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PTP1B Antibody (clone OTI1D10)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IF (1:100), IHC, IHC-P (1:150), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1D10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5770
  • Target name
    PTPN1
  • Target description
    protein tyrosine phosphatase non-receptor type 1
  • Target synonyms
    protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; PTP1B; tyrosine-protein phosphatase non-receptor type 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTP1B using anti-PTP1B antibody (A00613-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP1B antigen affinity purified polyclonal antibody (Catalog # A00613-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTP1B at approximately 57KD. The expected band size for PTP1B is at 50KD.
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