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ProductsBack/PXK Antibody (aa221-251, APC)

PXK Antibody (aa221-251, APC)

LS-C281714
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPXK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PXK Antibody (aa221-251, APC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    APC (Allophycocyanin)
  • Estimated Purity
    ...
  • Gene ID54899
  • Target name
    PXK
  • Target description
    PX domain containing serine/threonine kinase like
  • Target synonyms
    modulator of Na,K-ATPase long form; MONAKA; PX domain-containing protein kinase-like protein; PX ser/thr kinase v2; PX serine/threonine kinase; SLOB
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PXK using anti-PXK antibody (A07555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXK antigen affinity purified polyclonal antibody (Catalog # A07555-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PXK at approximately 65 kDa. The expected band size for PXK is at 65 kDa.
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