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ProductsBack/PYCARD / ASC / TMS1 Antibody (aa51-63)

PYCARD / ASC / TMS1 Antibody (aa51-63)

LS-C186594
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPYCARD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PYCARD / ASC / TMS1 Antibody (aa51-63)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 21 and 23kD bands observed in Mouse and Rat Spleen lysates respectively (calculated MW of 21.5kD according to Mouse NP_075747.3). Recommended concentration: 1-3 ug/ml.. Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 21 and 23kD bands observed in Mouse and Rat Spleen lysates respectively (calculated MW of 21.5kD according to Mouse NP_075747.3). Recommended concentration: 1-3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29108
  • Target name
    PYCARD
  • Target description
    PYD and CARD domain containing
  • Target synonyms
    apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain-containing protein 5; target of methylation-induced silencing 1; TMS; TMS1; TMS-1
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.
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