QuDye dsDNA HS Assay Kit

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QuDye dsDNA HS Assay Kit

7

Research Use Only

This kit for DNA quantification is optimized for Qubit® fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/microl to 100 ng/microl. The kit includes the concentrated QuDye HS assay reagent, dilution buffer, and DNA standards (0 and 10 ng/microl). The assay is selective towards dsDNA (double-stranded DNA) over RNA and is accurate for initial sample concentrations from 10 pg/microl to 100 ng/microl. This version of the kit also contains thin-walled tubes for use with Qubit™ fluorometers. The experiment is performed at room temperature and the signal is stable for 3 hours. The assay tolerates contaminants like salts, free nucleotides, detergents, or proteins. This kit simply requires the dilution of the reagents and buffer concentrates before you can start your measurements. The Qubit® instrument is able to read samples of about 200 microl. For sample preparation mix 180-199 microl of the QuDye HS working solution and 1-20 microl of the DNA sample to be quantified.

Store at 2-8°C. Warm to room temperature before use.

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