ICC/IF analysis of 3T3-L1 cells using GTX23337 RAB3D antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA incubated for 3 hours at room temperature
ICC/IF analysis of 3T3-L1 cells using GTX23337 RAB3D antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA incubated for 3 hours at room temperature
RAB3D antibody
GTX23337
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetRab3d
Overview
- SupplierGeneTex
- Product NameRAB3D antibody
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not detect rab 3A, 3B, or 3C.
- Application Supplier NoteWB: 2.0 microg/ml. ICC/IF: 2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID19340
- Target nameRab3d
- Target descriptionRAB3D, member RAS oncogene family
- Target synonymsC130057E11Rik; ras-related protein Rab-3D
- HostRabbit
- IsotypeIgG
- Protein IDP35276
- Protein NameRas-related protein Rab-3D
- Scientific DescriptionRab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
- ReactivityMouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203