Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1microg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
![RAB6 (Q72L mutant specific) antibody [AA2]](https://www.genetex.com/upload/website/prouct_img/normal/GTX33942/GTX33942_20160831_Structure_w_23060801_977.webp "RAB6 (Q72L mutant specific) antibody [AA2]")
Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2). Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1microg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
RAB6 (Q72L mutant specific) antibody [AA2]
GTX33942
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
ReactivityDrosophila, Human, Mouse
Overview
- SupplierGeneTex
- Product NameRAB6 (Q72L mutant specific) antibody [AA2]
- Delivery Days Customer9
- Antibody SpecificityRecognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6-GDP.
- Application Supplier NoteImmunocytochemistry: 1:1000
- ApplicationsImmunoFluorescence, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAA2
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostHuman
- IsotypeIgG2
- ReactivityDrosophila, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203