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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA994941(RAB9A Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA994941(RAB9A Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA994941(RAB9A Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

RAB9A Antibody

CSB-PA994941
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRAB9A
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Overview

  • Supplier
    Cusabio
  • Product Name
    RAB9A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9367
  • Target name
    RAB9A
  • Target description
    RAB9A, member RAS oncogene family
  • Target synonyms
    RAB9; RAB9, member RAS oncogene family; ras-related protein Rab-9A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51151
  • Protein Name
    Ras-related protein Rab-9A
  • Scientific Description
    Ras-related protein Rab-9A is a protein that in humans is encoded by the RAB9Agene. Rab 9A is a 201 amino acid protein that localizes to the cytoplasmic side of the cell membrane, as well as to the membrane of the Golgi apparatus and the ER, and is involved in the transport of proteins between endosomes and the trans Golgi network.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (A05768). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab9/RAB9A antigen affinity purified polyclonal antibody (Catalog # A05768) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab9/RAB9A at approximately 23KD. The expected band size for Rab9/RAB9A is at 23KD.
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Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37°C for 30min; Antibody incubation with RAB9 Polyclonal Antibody, Unconjugated bs-6177R at 1:400 overnight at 4°C, , followed by a conjugated secondary antibody and DAB staining.
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