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ProductsBack/Rabbit anti-CEACAM1 Recombinant Monoclonal Antibody [BLR240L]

Rabbit anti-CEACAM1 Recombinant Monoclonal Antibody [BLR240L]

A700-240CF
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCeacam1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-CEACAM1 Recombinant Monoclonal Antibody [BLR240L]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BLR240L
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26365
  • Target name
    Ceacam1
  • Target description
    carcinoembryonic antigen-related cell adhesion molecule 1
  • Target synonyms
    bb-1; Bg; Bgp; Bgp1; BGP-1; biliary glycoprotein 1; biliary glycoprotein D; carcinoembryonic antigen 1; carcinoembryonic antigen 7; carcinoembryonic antigen-related cell adhesion molecule 1; Cc1; C-CA; C-CAM; CD66; CD66a; Cea; Cea-; Cea1; Cea-1; Cea7; Cea-7; CEA-related cell adhesion molecule 1; hepatitis virus (MHV-4) susceptibility; hepatitis virus receptor; Hv; Hv-; Hv2; Hv-2; mCEA; mCEA1; MHV; Mhv-; Mhv-1; MHVR; MHV-R; MHVR1; mmCGM1; mmCGM1a; mmCGM2; murine hepatitis virus receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31809
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse Raw264.7 whole cell lysates, Lane 3: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 150 kDa. The expected band size for CEACAM1 is at 57,50,37,30 kDa.
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