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ProductsBack/Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT

Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT

X1589P
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetS1PR3
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (5-10 microg/ml) and Immunoprecipitation (see reference 4). Optimal concentration should be evaluated by serial dilutions. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    Western Blotting;Immunoprecipitation
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1903
  • Target name
    S1PR3
  • Target description
    sphingosine-1-phosphate receptor 3
  • Target synonyms
    bA791O21.3; C9orf108; C9orf47; EDG3; EDG-3; endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; sphingosine 1-phosphate receptor 3; sphingosine 1-phosphate receptor Edg-3; uncharacterized protein C9orf47
  • Host
    Rabbit
  • Protein IDQ99500
  • Protein Name
    Sphingosine 1-phosphate receptor 3
  • Scientific Description
    Endothelial cell differentiation gene 3 C-terminal; Sphingosine-1-Phosphate Receptor-3 (S1P3)
  • Shelf life instruction
    See expiration date on vial
  • Source
    Rabbits were immunized with a synthetic peptide derived from the C terminal of the EDG3 (S1P3) proteins
  • Reactivity Supplier
    Human;Mouse
  • UNSPSC
    12352203

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Lane 1: Rat Cerebrum cell lysates; Lane 2: Mouse Cerebrum cell lysates; Lane 3:Rat Heart cell lysates; Lane 4: Mouse Heart cell lysates probed with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of EDG3 using anti-EDG3 antibody (M03755-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG3 antigen affinity purified monoclonal antibody (Catalog # M03755-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG3 at approximately 42 kDa. The expected band size for EDG3 is at 42 kDa.
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