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Rabbit anti-KIF15 Antibody, Affinity Purified

A302-706A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetKIF15
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-KIF15 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID56992
  • Target name
    KIF15
  • Target description
    kinesin family member 15
  • Target synonyms
    BRDCS2, HKLP2, KLP2, KNSL7, NY-BR-62, kinesin-like protein KIF15, kinesin-like 7, kinesin-like protein 2, kinesin-like protein 7, serologically defined breast cancer antigen NY-BR-62
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS87
  • Protein Name
    Kinesin-like protein KIF15
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIF15 using anti-KIF15 antibody (A05983-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF15 antigen affinity purified polyclonal antibody (Catalog # A05983-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF15 at approximately 150-160KD. The expected band size for KIF15 is at 150-160KD.
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