Rabbit anti Mouse milk specific proteins

Nordic-MUbio

Rabbit anti Mouse milk specific proteins

7

Precipitating polyclonal Rabbit antiSerum to Mouse milk-specific proteins

Precipitation assays. In immunoelectrophoresis use 2 microl milk or equivalent against 120 microl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 microl antiserum in 3 mm diameter center well and 2 microl milk samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual protein components in individual milk samples. Immunohistochemical application: For immunostaining, paraffin-embedded sections are dehydrated and antigenic epitopes are exposed by treatment with 10mM citrate buffer for 5min at 121°C using an autoclave (see reference 1).

ImmunoElectrophoresis, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunoelectrophoresis;Immunohistochemistry (frozen & paraffin)

Primary antibodies

Research Use Only

Polyclonal

Rabbit

Pooled mouse milk. Freunds complete adjuvant is used in the first step of the immunization procedure.

Mouse

Mouse

-20°C,2°C to 8°C

12352203