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Rabbit anti Mouse Sphingosine Kinase 1 (pS225)

Research Use Only
X1849P
Nordic-MUbio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetSphk1
Price on request
100 ug
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti Mouse Sphingosine Kinase 1 (pS225)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (1-5microg/ml). Antibody shows some cross reactivity (~10% by ELISA) with other phosphoserine containing proteins. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Western Blotting;ELISA
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID20698
  • Target name
    Sphk1
  • Target description
    sphingosine kinase 1
  • Target synonyms
    1110006G24Rik; acetyltransferase SPHK1; SK; Sk1; sphingosine kinase 1; Spk1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8CI15
  • Protein Name
    Sphingosine kinase 1
  • Shelf life instruction
    See expiration date on vial
  • Source
    Rabbits were immunized with a synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • UNSPSC
    12352203

References

  • 1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585 2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-63 3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558 4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269 5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407 6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73 7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73 8. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459