Rabbit anti Mouse Sphingosine Kinase 1 (pS225)
X1849P
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetSphk1
Overview
- SupplierNordic-MUbio
- Product NameRabbit anti Mouse Sphingosine Kinase 1 (pS225)
- Delivery Days Customer7
- Application Supplier NoteAntibody can be used for Western blotting (1-5microg/ml). Antibody shows some cross reactivity (~10% by ELISA) with other phosphoserine containing proteins. Optimal concentration should be evaluated by serial dilutions.
- ApplicationsWestern Blot, ELISA
- Applications SupplierWestern Blotting;ELISA
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID20698
- Target nameSphk1
- Target descriptionsphingosine kinase 1
- Target synonyms1110006G24Rik; acetyltransferase SPHK1; SK; Sk1; sphingosine kinase 1; Spk1
- HostRabbit
- IsotypeIgG
- Protein IDQ8CI15
- Protein NameSphingosine kinase 1
- Shelf life instructionSee expiration date on vial
- SourceRabbits were immunized with a synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1
- ReactivityMouse
- Reactivity SupplierMouse
- UNSPSC12352203
References
- 1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585 2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-63 3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558 4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269 5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407 6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73 7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73 8. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459