WB analysis using Anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000.