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Figure 1. Model for generation of supercoiling by hRad54 translocation along DNA. The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick.
Figure 1. Model for generation of supercoiling by hRad54 translocation along DNA. The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick.
Figure 1. Model for generation of supercoiling by hRad54 translocation along DNA. The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick.

RAD54 (RAD54L) (1-17) Rabbit Polyclonal Antibody

AP09254PU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRAD54L
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    RAD54 (RAD54L) (1-17) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8438
  • Target name
    RAD54L
  • Target description
    RAD54 like
  • Target synonyms
    DNA repair and recombination protein RAD54-like; hHR54; HR54; hRAD54; RAD54 homolog; RAD54A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92698
  • Protein Name
    DNA repair and recombination protein RAD54-like
  • Scientific Description
    RAD54 (RAD54L) (1-17) rabbit polyclonal antibody, Aff - Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203