WB analysis of various samples using GTX01196 Raf1 antibody [GT1215]. Dilution : 1:1000 Loading : 25microg
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [GT1215] (GTX01196) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01196/GTX01196_4000000117_20200313_WB_TPM_watermark_w_23053121_953.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 antibody [GT1215] (GTX01196) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
WB analysis of various samples using GTX01196 Raf1 antibody [GT1215]. Dilution : 1:1000 Loading : 25microg
Raf1 antibody [GT1215] - Recombinant antibody, Orthogonal Validated
GTX01196
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAF1
Overview
- SupplierGeneTex
- Product NameRaf1 antibody [GT1215] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1215
- ConjugateUnconjugated
- Gene ID5894
- Target nameRAF1
- Target descriptionRaf-1 proto-oncogene, serine/threonine kinase
- Target synonymsCMD1NN; CRAF; c-Raf; C-Raf proto-oncogene, serine/threonine kinase; NS5; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203