Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Raf1-S338 pAb (TA380681) at 1:1000 dilution or Phospho-Raf1-S338 antibody (A0223).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 5s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Raf1-S338 pAb (TA380681) at 1:1000 dilution or Phospho-Raf1-S338 antibody (A0223).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 5s.
RAF1 Rabbit Polyclonal Antibody
TA380681
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAF1
Overview
- SupplierOriGene
- Product NameRAF1 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID5894
- Target nameRAF1
- Target descriptionRaf-1 proto-oncogene, serine/threonine kinase
- Target synonymsCMD1NN; CRAF; c-Raf; C-Raf proto-oncogene, serine/threonine kinase; NS5; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPhospho-Raf1-S338 Rabbit polyclonal Antibody
- ReactivityHuman, Mouse, Rat
- UNSPSC12352203