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ProductsBack/RAG1 antibody [1D9]
ELISA analysis of antigen using GTX60644 RAG1 antibody [1D9]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60644 RAG1 antibody [1D9]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60644 RAG1 antibody [1D9]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng

RAG1 antibody [1D9]

GTX60644
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetRAG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RAG1 antibody [1D9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1d9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5896
  • Target name
    RAG1
  • Target description
    recombination activating 1
  • Target synonyms
    RAG-1; recombination activating gene 1; recombination activating protein 1; RING finger protein 74; RNF74; V(D)J recombination-activating protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15918
  • Protein Name
    V(D)J recombination-activating protein 1
  • Scientific Description
    The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAG1 using anti-RAG1 antibody (A00307-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAG1 antigen affinity purified polyclonal antibody (Catalog # A00307-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAG1 at approximately 120KD. The expected band size for RAG1 is at 120KD.
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