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RALB Antibody

ORB1334014
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRALB
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Overview

  • Supplier
    Biorbyt
  • Product Name
    RALB Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB 1:200~4000, IHC 1:150, IF 1:100 IF, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2C4
  • Conjugate
    Unconjugated
  • Gene ID5899
  • Target name
    RALB
  • Target description
    RAS like proto-oncogene B
  • Target synonyms
    RALB Ras like proto-oncogene B; ras related GTP binding protein B; RAS-like protein B; ras-related protein Ral-B; v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11234
  • Protein Name
    Ras-related protein Ral-B
  • Scientific Description
    Mouse monoclonal antibody to RALB
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RALB using anti-RALB antibody (PB9795). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Thymus Tissue Lysate at 50ug, Lane 3: Rat Lung Tissue Lysate at 50ug, Lane 4: Mouse Spleen Tissue Lysate at 50ug, Lane 5: Mouse Liver Tissue Lysate at 50ug, Lane 6: HELA Whole Cell Lysate at 40ug, Lane 7: 22RV1 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALB antigen affinity purified polyclonal antibody (Catalog # PB9795) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALB at approximately 23 kDa. The expected band size for RALB is at 23 kDa.
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Western blot All lanes: RALB antibody at 1.26microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse lung tissue Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 24, 26 kDa Observed band size: 26 kDa
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