FACS analysis of HEK293T cells transfected with either RALBP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83730 RALBP1 antibody [6G10].
![IHC-P analysis of kidney tissue using GTX83730 RALBP1 antibody [6G10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_1769_IHC-P_w_23061420_415.webp "IHC-P analysis of kidney tissue using GTX83730 RALBP1 antibody [6G10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of liver tissue using GTX83730 RALBP1 antibody [6G10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_1770_IHC-P_w_23061420_280.webp "IHC-P analysis of liver tissue using GTX83730 RALBP1 antibody [6G10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IP analysis of DDDDK tagged RALBP1 overexpressed HEK293T lysate using GTX83730 RALBP1 antibody [6G10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_3392_IP_w_23061420_498.webp "IP analysis of DDDDK tagged RALBP1 overexpressed HEK293T lysate using GTX83730 RALBP1 antibody [6G10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83730 RALBP1 antibody [6G10]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_3757_WB_w_23061420_854.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83730 RALBP1 antibody [6G10]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with RALBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83730 RALBP1 antibody [6G10]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_3860_WB_w_23061420_504.webp "WB analysis of HEK293T cells transfected with RALBP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83730 RALBP1 antibody [6G10]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX83730 RALBP1 antibody [6G10]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_3862_WB_w_23061420_215.webp "WB analysis of various cell lines using GTX83730 RALBP1 antibody [6G10]. Loading : 35 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with RALBP1 plasmid using GTX83730 RALBP1 antibody [6G10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83730/GTX83730_796_ICCIF_w_23061420_347.webp "ICC/IF analysis of COS7 cells transiently transfected with RALBP1 plasmid using GTX83730 RALBP1 antibody [6G10].")
FACS analysis of HEK293T cells transfected with either RALBP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83730 RALBP1 antibody [6G10].
RALBP1 antibody [6G10]
GTX83730
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRALBP1
Overview
- SupplierGeneTex
- Product NameRALBP1 antibody [6G10] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6G10
- Concentration0.9 mg/ml
- ConjugateUnconjugated
- Gene ID10928
- Target nameRALBP1
- Target descriptionralA binding protein 1
- Target synonyms76 kDa Ral-interacting protein; dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; ralA-binding protein 1; ral-interacting protein 1; RIP1; RLIP1; RLIP76
- HostMouse
- IsotypeIgG2a
- Protein IDQ15311
- Protein NameRalA-binding protein 1
- Scientific DescriptionCan activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203