Detection of endogenous Raptor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20221PU-N Raptor antibody (Clone 10E10) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT-93 Lane 8: Neuro 2A Lane 9: 3T3
Detection of endogenous Raptor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20221PU-N Raptor antibody (Clone 10E10) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT-93 Lane 8: Neuro 2A Lane 9: 3T3
Raptor (RPTOR) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10E10]
AM20221PU-N
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetRPTOR
Overview
- SupplierOriGene
- Product NameRaptor (RPTOR) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10E10]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10E10
- Gene ID57521
- Target nameRPTOR
- Target descriptionregulatory associated protein of MTOR complex 1
- Target synonymsKOG1; Mip1; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats; raptor; regulatory-associated protein of mTOR
- HostMouse
- IsotypeIgG1
- Scientific DescriptionRaptor (RPTOR) (incl. pos. control) mouse monoclonal antibody, clone 10E10, Purified
- ReactivityCanine, Human, Mouse, Rat
- UNSPSC12352203