Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Biorbyt

Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

10

standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rat 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve

1.56-100 ng/mL

0.46 ng/mL

Research Use Only

Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

41116158